TISSUE ENGINEERING


TISSUE ENGINEERING

Multipotent vascular stem cells

Nuclear Alignment: Percentage of nuclei oriented greater than 80° away from strain direction for rMVSC (A,B,E,F) or RASMC (C,D,E,F) exposed to 1 Hz uniaxial cyclic tensile strain at 4-6% strain, 2-8% strain, 0-10% for 24 h (A,C,E) or 72 h (B,D,F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 # - Significantly different than 4-6% strained y - Significantly different from 2 to 8% strained z - Significantly different than 0-10% strained. Repeated symbols signify level of significance.


F-actin Alignment: Percentage of f-actin regions oriented greater than 80° away from strain direction for rMVSC (A,B,E,F) or RASMC (C,D,E,F) exposed to 1 Hz uniaxial cyclic tensile strain at 4-6% strain, 2-8% strain, 0-10% for 24 h (A,C,E) or 72 h (B,D,F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 # - Significantly different than 4-6% strained y - Significantly different from 2 to 8% strained z - Significantly different than 0-10% strained. Repeated symbols signify level of significance.

[1] P. Mathieu, E. Fitzpatrick, M. Di Luca, P. Cahill and C. Lally, "Resident multipotent vascular stem cells exhibit amplitude dependent strain avoidance similar to that of vascular smooth muscle cells", Biochemical and Biophysical Research Communications, vol. 521, no. 3, pp. 762-768, 2020. Available: 10.1016/j.bbrc.2019.10.185

Agent Based Modelling

Images of RASMC cultured on PDMS after 24 h (A,C) and 72 h (B,D) of no strain (A,B) or 0-10% 1 Hz cyclic tensile strain (C,D). Blue - DAPI nuclie, Red- Phalloidin f-actin.


Representative images of RASMC on deceullarized porcine carotid artery samples (A-D) left unstrained, (A,C) or strained parallel (B) or perpendicular (D) to the direction of collagen fibers. Blue - Nuclei, Red - F-actin.

[1] O.  McGee, D.  Nolan, P.  Mathieu and C.  Lally, "An in-silico Investigation Into the Role of Strain and Structure on Vascular Smooth Muscle Cell Growth", Frontiers in Bioengineering and Biotechnology, vol. 9, 2021. 10.3389/fbioe.2021.641794